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human renal proximal tubular epithelial cells rptecs  (ATCC)


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    ATCC human renal proximal tubular epithelial cells rptecs
    Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
    Human Renal Proximal Tubular Epithelial Cells Rptecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+renal+proximal+tubular+epithelial+cells+rptecs/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pm35791741-67-0-10
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    1) Product Images from "Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression."

    Article Title: Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.

    Journal: Kidney research and clinical practice

    doi: 10.23876/j.krcp.21.303

    Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.
    Figure Legend Snippet: Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Cell Culture:

    Article Title: Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.
    Article Snippet: .. Human renal proximal tubular epithelial cells (RPTECs) were cultured in American Type Culture Collection (ATCC)-formulated DMEM/F12 supplemented with an hTERT RPTEC Growth Kit (ATCC, Manassas, VA, USA) as previously described [21]. .. Total RNA was isolated from PKD2+/+, PKD2+/–, and PKD2–/– porcine fibroblasts using the TRIzol method according to the manufacturer’s protocol (Qiagen, Hilden, Germany).

    Article Title: Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression
    Article Snippet: .. Human renal proximal tubular epithelial cells (RPTECs) were cultured in American Type Culture Collection (ATCC)-formulated DMEM/F12 supplemented with an hTERT RPTEC Growth Kit (ATCC, Manassas, VA, USA) as previously described [ ]. .. Total RNA was isolated from PKD2 +/+ , PKD2 +/– , and PKD2 –/– porcine fibroblasts using the TRIzol method according to the manufacturer’s protocol (Qiagen, Hilden, Germany).



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    Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
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    Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of <t>RPTECs</t> and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular <t>epithelial</t> cell. *p < 0.05, ***p < 0.001.
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    Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.

    Journal: Kidney research and clinical practice

    Article Title: Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.

    doi: 10.23876/j.krcp.21.303

    Figure Lengend Snippet: Figure 3. Expression and secretion of OPN were reduced in renal cyst cells from patients with ADPKD. (A) mRNA expression anal ysis of RPTECs and two different renal cyst cells from ADPKD patients by real-time polymerase chain reaction. The relative expression of OPN in RPTECs and renal cyst cells was normalized to the expression of GAPDH. (B) OPN protein expression in RPTECs and renal cyst cells. Beta-actin was used as a control for Western blotting. (C) The level of OPN secreted from RPTECs and renal cyst cells was measured by ELISA. ADPKD, autosomal dominant polycystic kidney disease; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phos phate dehydrogenase; mRNA, messenger RNA; OPN, osteopontin; RPTEC, renal proximal tubular epithelial cell. *p < 0.05, ***p < 0.001.

    Article Snippet: Human renal proximal tubular epithelial cells (RPTECs) were cultured in American Type Culture Collection (ATCC)-formulated DMEM/F12 supplemented with an hTERT RPTEC Growth Kit (ATCC, Manassas, VA, USA) as previously described [21].

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot, Enzyme-linked Immunosorbent Assay